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OLGICA BAKAJIN |
Transverse Pulse-Field DNA Fractionation In Microfabricated Arrays |
Keywords: DNA, DNA fractionation, polymer dynamics in reduced dimensions,
microfabrication, electrophoresis |
Application of mathematics and physics together with microfabrication
technology to problems in molecular biology promises to result in DNA fractionation
technique with better resolution that would also be faster, cheaper and
would increase the range of lengths of DNA that can be fractionated. As
a restrictive medium we propose very regular and reproducible microfabricated
solid-state arrays. By using numerical simulations we can model motion
of the DNA in these quasi-two-dimensional devices and through modeling
optirnize the design and experimental parameters. We propose to build hexagonal
arrays of obstacles in which we can perforrn DNA fractionation by transverse
pulse-field electrophoresis. |
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